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anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody
    Anti Engrailed 4d9 (1:5, Dshb, Ia) Mouse Primary Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-engrailed+4d9/monoclonal+anti+engrailed+antibody+4d9/bio_rxiv__2025__07__02__662832-340-16-19
    Average 90 stars, based on 1 article reviews
    anti-engrailed 4d9 (1:5, dshb, ia) mouse primary antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Next-Generation Sequencing:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Staining:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Marker:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Clone Assay:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    CRISPR:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Labeling:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Sequencing:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Recombinant:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Solvent:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Software:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.

    Knock-In:

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: The neuropil was stained with mouse anti-engrailed (RRID: AB_528224 ) (DSHB, 4D9) and Alexa Fluor 647 Phalloidin (ThermoFisher) by following the manufacturer’s protocol.

    Article Title: Sclerotome is compartmentalized by parallel Shh and Bmp signaling downstream of CaMKII
    Article Snippet: Embryos were fixed in 4% formaldehyde and incubated with rabbit anti-phospho-Smad-1,5,8/9 (Cell Signaling, 13820, 1:20), rabbit anti-γ1/δ2 CaMKII (GeneTex, GTX128842, 1:20), rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech, 1:20), mouse anti-engrailed (Developmental Studies Hybridoma Bank, University of Iowa (4D9) 1:100), mouse anti-myosin, sarcomere (Developmental Studies Hybridoma Bank, University of Iowa (MF20) 1:100), or mouse anti-myosin heavy chain, slow muscle (Developmental Studies Hybridoma Bank, University of Iowa (F59) 1:100).

    Article Title: A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
    Article Snippet: DOI: https://doi.org/10.7554/eLife.67510 20 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Drosophila melanogaster) w[1118] P{y[+t7.7] w[+mC]=R57 C10FLPG5.PEST} attP18; P{y[+t7.7] w[+mC]=10xUAS(FRT. stop)myr::smGdP-OLLAS} attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop) myr::smGdP-HA}VK00005 P{10xUAS(FRT. stop) myr::sm GdP-V5-THS-10xUAS (FRT.stop)myr::smGdPFLAG}su(Hw)attP1 BDSC RRID:BDSC_64091 Multicolor Flp Out stock (Nern et al., 2015) Genetic reagent (Drosophila melanogaster) Sco/CyO; Dr/TM3,Sb BDSC RRID:BDSC_34516 Genetic reagent (Drosophila melanogaster) LexA-T2A-Hb This work Endogenous hb locus CRISPR engineered to place LexAp65-T2A upstream and in frame with the first hb ORF Genetic reagent (Drosophila melanogaster) hey:T2A:FLP, UAS-myr:GFP, UAS-FRT-stopFRT-myr:sfGdP:HA This work Labels Hey+ (NotchON) neurons within a Gal4+ neuronal population Antibody, polyclonal Rabbit antiGFP A-11122 ThermoFisher, Waltham, MA RRID:AB_221569 1:500 Antibody, polyclonal Chicken anti-GFP Abcam, Eugene, OR RRID:BDSC_13970 1:1000 Antibody, polyclonal Camelid sdAB direct labeled with AbberiorStar635P ‘Fluo Tag-Q anti-GFP’ #N0301 NanoTab Biotech., Gottingen, Germany 1:1000 Antibody, polyclonal Rabbit anti-mCherry NBP2-25157 Novus, Littleton, CO RRID:AB_2753204 1:1000 Antibody, polyclonal Alexa Fluor 488- conjugated rabbit anti-GFP NBP1-69969 ThermoFisher (Eugene, OR) RRID:AB_221477 1:1000 Antibody, monoclonal Mouse anti-FasII 1D4 DSHB (Iowa City, IA) RRID:AB_528235 1:100 Antibody, monoclonal Mouse antiHA(6E2) #2350 Cell Signaling, Danvers, MA RRID:AB_491023 1:200 Antibody, polyclonal Rabbit anti-V5 Dylite 549 #600-442-378 Rockland, Atlanta, GA RRID:AB_1961802 1:400 Antibody, polyclonal Rabbit anti-FLAG Dylite488 # 600- 441-383 Rockland, Atlanta, GA RRID:AB_1961508 1:200 Antibody, monoclonal Mouse antiEngrailed 4D9 DSHB (Iowa City, IA) RRID:AB_528224 1:100 Antibody, polyclonal Rabbit anti-Hb Doe lab 1:400 Antibody, polyclonal Alexa Fluor 405 Phalloidin ThermoFisher (Eugene, OR) 1:40 Antibody, polyclonal Secondary antibodies ThermoFisher (Eugene, OR) 1:400 Continued on next page Mark et al. eLife 2021;10:e67510.

    Article Title: Doublesex controls specification and maintenance of the gonad stem cell niches in Drosophila
    Article Snippet: Fisher, State University of New York, NY, USA) at 1:20, mouse anti-Engrailed 4D9 (DSHB; C. Goodman) at 1:2, rabbit anti-phospho histone H3 (Upstate) at 1:5000, rabbit anti-BAB1 (S. Carroll), rat anti-BAB2 R10 (F. Laski), mouse anti-armadillo N2 7A1 (DSHB; E. Wieschaus, Princeton University, NJ, USA).

    Article Title: Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
    Article Snippet: Mouse anti-Engrailed (DSHB, 4D9), 1:50.

    Article Title: Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.
    Article Snippet: Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs.. A mutation in the enzyme’s catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity.. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis.

    Article Title: Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ
    Article Snippet: Primary antibodies and the concentrations used to stain the larval-pupal transition stage ovaries are as follows: Mouse anti-Engrailed 4D9 (1:40, Developmental Studies Hybridoma Bank (DSHB)), Rabbit anti-Vasa (1:3000, gift from A. Nakamura, Kumamoto University), Rat anti-Chinmo (1:500, gift from E. Bach, New York University; (Wu 2012).

    Article Title: Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress
    Article Snippet: Mouse anti-Engrailed , Developmental Studies Hybridoma Bank , 4D9; RRID: AB_528224.



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    Image Search Results


    Journal: microPublication Biology

    Article Title: Identification of a novel downstream single-minded midline regulatory element in Drosophila melanogaster

    doi: 10.17912/micropub.biology.001317

    Figure Lengend Snippet:

    Article Snippet: For fluorescent staining, primary antibodies were mouse anti-Slit C555.6D (1:10; Developmental Studies Hybridoma Bank), mouse anti-Nubbin 2D4 (1:10, DSHB), mouse anti-Engrailed 4D9 (DSHB), and Mab 22C10 (1:20, DSHB).

    Techniques: Sequencing, Recombinant, Plasmid Preparation